Home
Resources
Publications
Determination of the Antioxidant 3-tert.-butyl-4-hydroxy-anisole in Rat-Plasma Using High-Resolution Gas Chromatography-Mass Spectrometry

Determination of the Antioxidant 3-tert.-butyl-4-hydroxy-anisole in Rat-Plasma Using High-Resolution Gas Chromatography-Mass Spectrometry

E Bailey, L Della Corte, P B Farmer, A J Gray

J Chromatogr. 1981 Sep 11;225(1):83-9.

PMID: 7298761

Abstract:

A method is described for the determination of the antioxidant 3-tert.-butyl-4-hydroxy-anisole in rat plasma using high-resolution capillary gas chromatography-mass spectrometry with selective ion monitoring. Following the addition of the isomer 2-tert.-butyl-4-hydroxy-anisole, used as an internal standard, extraction was made with n-hexane and the extract derivatized with heptafluorobutyric anhydride. The gas chromatographic separation was carried out on a SE-52 fused silica capillary column and the derivatized 3-tert.-butyl-4-hydroxyanisole and its isomer detected by recording the intensities of their common fragment ion at m/e 361. The sensitivity of the method allowed the antioxidant to be measured in 0.1-ml rat plasma samples down to a level of 10 ng/ml with a high degree of specificity and accuracy. The method has been applied to a preliminary pharmacokinetic study in rats after oral dosage.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP121006-A	3-tert-Butyl-4-hydroxyanisole		121-00-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: