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Determination of the Molecular-Weight Distribution of Low-Molecular-Weight Heparins Using High-Performance Gel Permeation Chromatography

Determination of the Molecular-Weight Distribution of Low-Molecular-Weight Heparins Using High-Performance Gel Permeation Chromatography

H Komatsu, T Takahata, M Tanaka, S Ishimitsu, S Okada

Biol Pharm Bull. 1993 Dec;16(12):1189-93.

PMID: 8130765

Abstract:

The molecular-weight distribution of commercially available low-molecular-weight heparins (LMWHs) was estimated by high-performance gel permeation chromatography (HPGPC) using a LMWH molecular-weight calibrant, supplied by the National Institute for Biological Standards and Control for the calibration of columns. This calibrant is a mixture of many polysaccharides with a disaccharide unit prepared by a random depolymerization of unfractionated heparin, and the ends of the fragments have a UV absorption. The ratio of the refractive index to the UV absorption is a relative measure of molecular weight. Various molecular-weight parameters such as weight-, number- or z-average molecular-weights, polydispersity and relative distributions of high, middle and low molecular-weight components were estimated, indicating that LMWHs have a wide variation in molecular-weight distribution. This variation was ascribed to the difference in preparation methods from unfractionated heparins.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9041081-G	Heparin Sodium Molecular Weight Calibrant		9041-08-1	Price
AP9041081-J	Low Molecular Weight Heparin Molecular Weight Calibrant		9041-08-1	Price

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