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[Determination of Urinary Oxalate Using a Sigma Kit]

[Determination of Urinary Oxalate Using a Sigma Kit]

K Suzuki, C Domiki

Hinyokika Kiyo. 1987 May;33(5):794-8.

PMID: 3661346

Abstract:

Many methods for measuring urinary oxalate have been reported. However, most of them are unsuitable for clinical use because of complexity and difficulty. We tested the value of the Sigma kit for measuring urinary oxalate. In principle, after extraction from urine, oxalate is oxidized to hydrogen peroxide in the presence of oxalate oxidase and the hydrogen peroxide reacts with 3-methyl-2 benzothiozolinone hydrazone and 3-(dimethylamino) benzoic acid in the presence of peroxidase to yield an indamine dye with a maximum absorbance at 590 nm. The linearity of the standard curve and reproducibility of this method were measured and good results were obtained. (Linearity: r = 0.998, within assay: C.V. = 1.5 to 5.0%, between assays: C.V. = 4.0 to 11.6%). The correlation between this method and modified Hodgkinson and Williams' method or ion chromatographic method were 0.922 and 0.796, respectively. The overestimation effect of added ascorbic acid was completely inhibited by FeCl3 which had been added beforehand.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP99649	3-(Dimethylamino)benzoic acid		99-64-9	Price

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