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Development and Characterization of Cross-Linked Enzyme Aggregates of Thermotolerant Alkaline Protease From Bacillus Licheniformis

Development and Characterization of Cross-Linked Enzyme Aggregates of Thermotolerant Alkaline Protease From Bacillus Licheniformis

Fareeha Bashir, Muhammad Asgher, Fatima Hussain, Muhammad Atif Randhawa

Int J Biol Macromol. 2018 Jul 1;113:944-951.

PMID: 29510168

Abstract:

An alkaline protease was produced by B. licheniformis with 132.43±3.4U/mL activity in LSF which was further enhanced by optimizing culture conditions. The optimum enzyme activity (148.9±4.1U/mL) was harvested at pH7.5; temperature, 40°C and inoculum, 1.5mL after 48h incubation. Alkaline protease was immobilization by forming cross linked enzyme aggregates (CLEAs) and the processes of CLEAs formation was also optimized. The protease CLEAs developed using 80% ammonium sulfate, 65mM glutaraldehyde and 0.11mM BSA showed best activity recovery (39.76%). Free protease and CLEAs were characterized and compared. It was observed that CLEAs of protease exhibited broad pH range with best activity at pH10. The immobilized protease was also thermo-tolerant with optimum activity at 65°C temperature. The Vmax and Km of protease-CLEAs were 125.5U/mL and 18.97μM, respectively as compared to 104.9U/mL and 29μM, respectively for free protease. It was concluded that immobilized enzyme in the form of CLEAs is a valuable catalyst for potential industrial applications.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9014011-B	Protease from Bacillus licheniformis		9014-01-1	Price

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