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Development and Validation of an LC-ESI-MS/MS Method for the Quantitation of Hemslecin A in Rhesus Monkey Plasma and Its Application in Pharmacokinetics

Development and Validation of an LC-ESI-MS/MS Method for the Quantitation of Hemslecin A in Rhesus Monkey Plasma and Its Application in Pharmacokinetics

Min Bai, Hong-Liang Li, Jian-Chang He, Gong-Hao He, En-Fu Feng, Yue-Qiong Liu, Pan-Pan Shi, Gui-Li Xu

Biomed Chromatogr. 2014 Mar;28(3):385-90.

PMID: 24132644

Abstract:

In this study, a new LC-ESI-MS/MS-based method was validated for the quantitation of hemslecin A in rhesus monkey plasma using otophylloside A as internal standard (IS). Hemslecin A and the IS were extracted from rhesus monkey plasma using liquid-liquid extraction as the sample clean-up procedure, and were subjected to chromatography on a Phenomenex Luna CN column (150 × 2.0 mm, 3.0 µm) with the mobile phase consisting of methanol and 0.02 mol/mL ammonium acetate (55:45, v/v) at a flow rate of 0.2 mL/min. Detection was performed on an Agilent G6410B tandem mass spectrometer by positive ion electrospray ionization in multiple reaction monitoring mode, monitoring the transitions m/z 580.5 [M + NH4 ](+) → 503.4 and m/z 518.2 [M + NH4 ](+) → 345.0 for hemslecin A and IS, respectively. The assay was linear over the concentration range of 0.5-200 ng/mL and was successfully applied to a pharmacokinetic study in rhesus monkeys.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP58546342	Hemslecin A		58546-34-2	Price

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