Home
Resources
Publications
Development of a Dopaminergic System in Sea Urchin Embryos and Larvae

Development of a Dopaminergic System in Sea Urchin Embryos and Larvae

Hideki Katow, Takashi Suyemitsu, Shio Ooka, Junko Yaguchi, Takayuki Jin-Nai, Iku Kuwahara, Tomoko Katow, Shunsuke Yaguchi, Hirokazu Abe

J Exp Biol. 2010 Aug 15;213(Pt 16):2808-19.

PMID: 20675551

Abstract:

The mechanisms that regulate the organized swimming movements of sea urchin blastulae are largely unknown. Using immunohistochemistry, we found that dopamine (DA) and the Hemicentrotus pulcherrimus homolog of the dopamine receptor D1 (Hp-DRD1) were strongly co-localized in 1-2 microm diameter granules (DA/DRD1 granules). Furthermore, these granules were arranged across the entire surface of blastulae as they developed locomotory cilia before hatching, and remained evident until metamorphosis. DA/DRD1 granules were associated with the basal bodies of cilia, and were densely packed in the ciliary band by the eight-arm pluteus stage. The transcription of Hp-DRD1 was detected from the unfertilized egg stage throughout the period of larval development. Treatment with S-(-)-carbidopa, an inhibitor of aromatic-l-amino acid decarboxylase, for 20-24 h (i) from soon after insemination until the 20 h post-fertilization (20 hpf) early gastrula stage and (ii) from the 24 hpf prism larva stage until the 48 hpf pluteus stage, inhibited the formation of DA granules and decreased the swimming activity of blastulae and larvae in a dose-dependent manner. Exogenous DA rescued these deprivations. The formation of DRD1 granules was not affected. However, in 48 hpf plutei, the serotonergic nervous system (5HT-NS) developed normally. Morpholino antisense oligonucleotides directed against Hp-DRD1 inhibited the formation of DRD1 granules and the swimming of larvae, but did not disturb the formation of DA granules. Thus, the formation of DRD1 granules and DA granules occurs chronologically closely but mechanically independently and the swimming of blastulae is regulated by the dopaminergic system. In plutei, the 5HT-NS closely surrounded the ciliary bands, suggesting the functional collaboration with the dopaminergic system in larvae.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP28860959	S-(−)-Carbidopa		28860-95-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: