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Development of a Highly Sensitive Chemiluminescent Assay for Hydrogen Peroxide Under Neutral Conditions Using Acridinium Ester and Its Application to an Enzyme Immunoassay

Development of a Highly Sensitive Chemiluminescent Assay for Hydrogen Peroxide Under Neutral Conditions Using Acridinium Ester and Its Application to an Enzyme Immunoassay

Hidetoshi Arakawa, Keiko Tsuruoka, Ken-ichi Ohno, Noriko Tajima, Hiromi Nagano

Luminescence. 2014 Jun;29(4):374-7.

PMID: 23832789

Abstract:

We developed a highly sensitive chemiluminescent (CL) assay for hydrogen peroxide using 10-methyl-9-(phenoxycarbonyl) acridinium fluorosulfonate (PMAC) that produced chemiluminescence under neutral conditions and applied it to an enzyme immunoassay (EIA). One picomole of hydrogen peroxide could be detected using the optimized PMAC-CL method and 6.2 × 10(-20) mol β-D-galactosidase (β-gal) could be detected by combining an indoxyl derivative substrate and the proposed PMAC-CL method. This highly sensitive CL β-gal assay was applied to an EIA for thyroid-stimulating hormone (TSH) using β-gal as a label enzyme; 0.02-100.0 μU/mL TSH in human serum could be assayed directly and with high reproducibility.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP149300549	10-Methyl-9-(phenoxycarbonyl)acridinium fluorosulfonate		149300-54-9	Price

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