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Development of a Method for the Separation of Corrinoids in Ovine Tissues by HPLC

Development of a Method for the Separation of Corrinoids in Ovine Tissues by HPLC

R J Kelly, T M Gruner, A R Sykes

Biomed Chromatogr. 2005 May;19(4):329-33.

PMID: 15828057

Abstract:

A method has been developed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to routinely estimate the distribution of corrinoids (the cobalamins hydroxocobalamin, methylcobalamin and 5'-deoxyadenosylcobalamin, and cobalamin analogues) in liver, plasma, milk, intestinal fluid and faeces. Corrinoids were extracted with a sodium acetate buffer, separated by HPLC and quantified by RIDA. Recoveries of corrinoids were 29% for hydroxocobalamin, 50% for 5'-deoxyadenosylcobalamin and 64% for methylcobalamin. The method allows the routine analysis of many samples and maintains good standards of precision.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP22465481	Hydroxocobalamin acetate		22465-48-1	Price

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