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Development of a Novel Functional Monoclonal Antibody to Human CD275: Characterization and Biological Activity

Development of a Novel Functional Monoclonal Antibody to Human CD275: Characterization and Biological Activity

Xiaohan Hu, Cuiping Liu, Jingnan An, Yu Shen, Yumin Hu, Juean Jiang, Jian Wu, Xueguang Zhang

Monoclon Antib Immunodiagn Immunother. 2016 Feb;35(1):18-24.

PMID: 26788906

Abstract:

CD275 (B7-H2, ICOSL), a co-stimulatory molecule of the B7 superfamily, plays a critical role in immune response. In this report, a novel mouse anti-human CD275 monoclonal antibody (MAb) was prepared using hybridoma technology, and immunological characteristics of the MAb were determined. The results showed that the MAb (clone 13D11) was IgG2(κ) and bound specifically to human CD275. By mutual competition, we found that the antibody recognized different epitopes of CD275 antigen compared with commercial antibodies and could block ICOS-CD275 interaction. Crosslinking of CD275 with MAb 13D11 markedly blocked ICOS positive signal and inhibited T cell proliferation and cytokine production. In addition, the 13D11 MAb was suitable for indirect ELISA detection. Thus, the MAb against human CD275 with high specificity and different activity would be useful for further study of this molecule.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413190	ICOSLG/B7-H2/CD275 human			Price

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