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Development of a Novel Monoclonal Antibody to Human Inducible Co-Stimulator Ligand (ICOSL): Biological Characteristics and Application for Enzyme-Linked Immunosorbent Assay

Development of a Novel Monoclonal Antibody to Human Inducible Co-Stimulator Ligand (ICOSL): Biological Characteristics and Application for Enzyme-Linked Immunosorbent Assay

Xiaohan Hu, Jian Wu, Jingnan An, Yumin Hu, Yu Shen, Cuiping Liu, Xueguang Zhang

Int Immunopharmacol. 2016 Jul;36:151-157.

PMID: 27138044

Abstract:

ICOSL (B7-H2, CD275), a co-stimulatory molecule of the B7 superfamily, functions as a positive signal in immune response. To investigate whether ICOSL could be released into sera and the possible biological function of soluble ICOS (sICOSL), we generated and characterized a functional anti-human ICOSL monoclonal antibody (mAb), 20B10, and developed a novel enzyme-linked immunosorbent assay (ELISA) based on two anti-human ICOSL antibodies with different epitope specificities. Using the ELISA system, we found that sICOSL in the serum of healthy donors increases in an age-dependent manner and that the matrix metalloproteinase inhibitor (MMPI) could suppress sICOSL production. Together, these data demonstrate that the existence of circulating sICOSL in human serum might play an important role in immunoregulation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413190	ICOSLG/B7-H2/CD275 human			Price

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