Home
Resources
Publications
Development of an Ionic Liquid-Based Ultrasonic-Assisted Liquid-Liquid Microextraction Method for Sensitive Determination of Biogenic Amines: Application to the Analysis of Octopamine, Tyramine and Phenethylamine in Beer Samples

Development of an Ionic Liquid-Based Ultrasonic-Assisted Liquid-Liquid Microextraction Method for Sensitive Determination of Biogenic Amines: Application to the Analysis of Octopamine, Tyramine and Phenethylamine in Beer Samples

Ke-Jing Huang, Chun-Xue Jin, Shi-Lin Song, Cai-Yun Wei, Yan-Ming Liu, Jing Li

J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Mar 15;879(9-10):579-84.

PMID: 21316314

Abstract:

A simple and efficient method, ionic liquid-based ultrasound-assisted liquid-liquid microextraction, has been developed for the determination of three biogenic amines including octopamine (OCT), tyramine (TYR) and phenethylamine (PHE). Fluorescence probe 2,6-dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester was applied for derivatization of biogenic amines and high-performance liquid chromatography coupled with fluorescence detection was used for the determination of the derivatives. The factors affecting the extraction efficiency, such as the type and volume of ionic liquid, ultrasonication time and centrifugation time have been investigated in detail. Under the optimum conditions, linearity of the method was observed in the range of 0.5-50 μgmL(-1) for OCT and TYR, and 0.025-2.5 μgmL(-1) for PHE, respectively, with correlation coefficients (γ)>0.996. The limits of detection ranged from 0.25-50 ngmL(-1) (S/N=3). The spiked recoveries of three target compounds in beer samples were in the range of 90.2-114%. As a result, this method has been successfully applied for the sensitive determination of OCT, TYR and PHE in beer samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP569355302	2,6-Dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester		569355-30-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: