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Development of an LC-MS/MS Method for the Analysis of Free Sphingoid Bases Using 4-fluoro-7-nitrobenzofurazan (NBD-F)

Development of an LC-MS/MS Method for the Analysis of Free Sphingoid Bases Using 4-fluoro-7-nitrobenzofurazan (NBD-F)

Toshiki Ishikawa, Hiroyuki Imai, Kawai-Yamada Maki

Lipids. 2014 Mar;49(3):295-304.

PMID: 24310230

Abstract:

The molecular species of sphingoid bases were tagged with the fluorescent amino group reagent, 4-fluoro-7-nitrobenzofurazan (NBD-F). The NBD-sphingoid bases were analyzed by a highly selective and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) technique capable of reliable detection of several fmol of the derivatives. Lipid extracts from plant samples were derivatized with NBD-F, and all nine species of free sphingoid bases present in plant sphingolipids were separated and quantified for the first time; a complete baseline resolution was achieved for cis-8 and trans-8 isomers of sphingoid bases by reversed phase HPLC on a C18 column. The extraction and derivatization procedures and LC-MS/MS method can facilitate the progress of the studies for seeking the active components of sphingoid bases species in response to biological challenges.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP29270562	4-Fluoro-7-nitrobenzofurazan		29270-56-2	Price

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