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Development of Biosensors Containing Laccase and Imidazolium Bis(trifluoromethylsulfonyl)imide Ionic Liquid for the Determination of Rutin

Development of Biosensors Containing Laccase and Imidazolium Bis(trifluoromethylsulfonyl)imide Ionic Liquid for the Determination of Rutin

Ana Cristina Franzoi, Pedro Migowski, Jairton Dupont, Iolanda Cruz Vieira

Anal Chim Acta. 2009 Apr 20;639(1-2):90-5.

PMID: 19345764

Abstract:

Biosensors based on hydrophobic ionic liquids (ILs) derived from the bis(trifluoromethylsulfonyl)imide [(CF(3)SO(2))(2)N(-) = Tf(2)N(-)] anion associated with three different imidazolium cations: 1-butyl-3-methylimidazolium (BMI x Tf(2)N), 1-decyl-3-methylimidazolium (DMI x Tf(2)N) and 1-tetradecyl-3-methylimidazolium (TDMI x Tf(2)N), along with laccase from Aspergillus oryzae, were constructed and optimized for determination of rutin. The laccase catalyzes the oxidation of rutin to the corresponding o-quinone, which is electrochemically reduced back to rutin. The best performance was obtained with 50:20:15:15% (w/w/w/w) as the graphite powder:laccase:Nujol:ILs composition in 0.1 mol L(-1) acetate buffer solution (pH 5.0). The parameters for the square-wave voltammetry experiments and scanning electron microscopy images of the biosensors were studied. Under the selected conditions, the cathodic peak current increased linearly in the rutin concentration ranges of 4.77x10(-6) to 4.62x10(-5) mol L(-1), 5.84x10(-6) to 5.36x10(-5) mol L(-1) and 5.84x10(-6) to 5.36x10(-5) mol L(-1) using the (I) BMI x Tf(2)N-laccase, (II) DMI x Tf(2)N-laccase and (III) TDMI x Tf(2)N-laccase, respectively. The rutin contents of commercial samples of pharmaceuticals were successfully determined by the biosensors and the results compared well with those obtained using the official method. The studies on rutin recovery from these samples gave values of 96.9-104.6%.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1017254635	1,3-Dimethoxy-2-methylimidazolium bis(trifluoromethylsulfonyl)imide		1017254-63-5	Price

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