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Development of ELISAs for the Detection of Vitellogenin in Three Marine Fish From Coastal Areas of China

Development of ELISAs for the Detection of Vitellogenin in Three Marine Fish From Coastal Areas of China

Yuejiao Li, Jun Wang, Mingyi Zheng, Yabin Zhang, Shaoguo Ru

Mar Pollut Bull. 2018 Aug;133:415-422.

PMID: 30041330

Abstract:

Estrogenic pollution has aroused great concern for its adverse effects on marine organisms. This study aimed to establish biomarker-based methods for detecting environmental estrogens using vitellogenin (Vtg) of teleost fishes inhabiting coastal areas of China. Firstly, Vtgs in marbled flounder (Pseudopleuronectes yokohamae), black rockfish (Sebastes schlegelii) and fat greenling (Hexagrammos otakii) were purified, characterized and used to prepare antibodies. Then, Vtg ELISA for each species was developed using purified Vtg and its antibody. Marbled flounder Vtg ELISA had a working range of 3.9-500 ng/mL and a detection limit of 2.1 ng/mL, and black rockfish Vtg ELISA had strong cross-reactivity with marbled flounder Vtg. Furthermore, Vtg induction in male marbled flounder exposed to pentadecafluorooctanoic acid (PFOA) was measured by developed ELISA. Plasma Vtg concentrations were significantly increased with PFOA concentrations in seawater and fish muscle. Therefore, Vtg ELISAs for these species might be useful tools for monitoring marine environmental estrogens.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP335671-A	Pentadecafluorooctanoic acid		335-67-1	Price

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