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Development of Reagents for Differential Protein Quantitation by Subtractive Parent (Precursor) Ion Scanning

Development of Reagents for Differential Protein Quantitation by Subtractive Parent (Precursor) Ion Scanning

Asa Wåhlander, Giorgio Arrigoni, Kristofer Wårell, Fredrik Levander, Ronnie Palmgren, Jean-Luc Maloisel, Philippe Busson, Peter James

J Proteome Res. 2007 Mar;6(3):1101-13.

PMID: 17286425

Abstract:

We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9048468-C	BSA for protein quantitation		9048-46-8	Price

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