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Differential Association of Products of Alternative Transcripts of the Candidate Tumor Suppressor ING1 With the mSin3/HDAC1 Transcriptional Corepressor Complex

Differential Association of Products of Alternative Transcripts of the Candidate Tumor Suppressor ING1 With the mSin3/HDAC1 Transcriptional Corepressor Complex

D Skowyra, M Zeremski, N Neznanov, M Li, Y Choi, M Uesugi, C A Hauser, W Gu, A V Gudkov, J Qin

J Biol Chem. 2001 Mar 23;276(12):8734-9.

PMID: 11118440

Abstract:

The candidate tumor suppressor ING1 was identified in a genetic screen aimed at isolation of human genes whose expression is suppressed in cancer cells. It may function as a negative growth regulator in the p53 signal transduction pathway. However, its molecular mechanism is not clear. The ING1 locus encodes alternative transcripts of p47(ING1a), p33(ING1b), and p24(ING1c). Here we report differential association of protein products of ING1 with the mSin3 transcriptional corepressor complex. p33(ING1b) associates with Sin3, SAP30, HDAC1, RbAp48, and other proteins, to form large protein complexes, whereas p24(ING1c) does not. The ING1 immune complexes are active in deacetylating core histones in vitro, and p33(ING1b) is functionally associated with HDAC1-mediated transcriptional repression in transfected cells. Our data provide basis for a p33(ING1b)-specific molecular mechanism for the function of the ING1 locus.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4246395	RbAp48 Active human			Price

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