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Differential Binding of Nuclear c-ets-1 Protein to an Intron I Fragment of the C-Myb Gene in Growth Versus Differentiation

Differential Binding of Nuclear c-ets-1 Protein to an Intron I Fragment of the C-Myb Gene in Growth Versus Differentiation

L G Wang, X M Liu, Z R Li, S Denstman, A Bloch

Cell Growth Differ. 1994 Nov;5(11):1243-51.

PMID: 7848925

Abstract:

For growth, ML-1 human myeloblastic leukemia cells require insulin-like growth factor 1 together with transferrin, whereas for differentiation they depend upon transforming growth factor beta in combination with transferrin. As shown in this study, growth stimulation is accompanied by c-myb expression, whereas initiation of differentiation results in the cessation of c-myb expression through premature termination of transcription in the first intron of the myb gene. Growth factor-stimulated c-myb elongation was found to correlate with an elevated level of nuclear c-ets-1 protein and with increased binding of this protein to an 18-base pair sequence in intron 1 of the c-myb gene containing the putative regulatory element PEA 3. In contrast, differentiation factor-initiated ML-1 cell maturation was accompanied by a very low level of nuclear c-ets-1 protein, by the inability to detect binding of the protein to the 18-base pair sequence, and by the cessation of c-myb expression. These results show a correlation to exist between c-ets-1 binding to intron 1 of the c-myb gene and c-myb expression. The mechanism underlying this correlation is under further study.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413303	IGF-Binding Protein 1 human			Price

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