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Differential Involvement of Signaling Pathways in the Regulation of Growth Hormone Release by Somatostatin and Growth Hormone-Releasing Hormone in Orange-Spotted Grouper (Epinephelus Coioides)

Differential Involvement of Signaling Pathways in the Regulation of Growth Hormone Release by Somatostatin and Growth Hormone-Releasing Hormone in Orange-Spotted Grouper (Epinephelus Coioides)

Bin Wang, Chaobin Qin, Cong Zhang, Jirong Jia, Caiyun Sun, Wensheng Li

Mol Cell Endocrinol. 2014 Feb 15;382(2):851-9.

PMID: 24183819

Abstract:

Somatostatin is the most effective inhibitor of GH release, and GHRH was recently identified as one of the primary GH-releasing factors in teleosts. In this study, we analyzed the possible intracellular transduction pathways that are involved in the mechanisms induced by SRIF and GHRH to regulate GH release. Using a pharmacological approach, the blockade of the PLC/IP/PKC pathway reversed the SRIF-induced inhibition of GH release but did not affect the GHRH-induced stimulation of GH release. Furthermore, SRIF reduced the GH release induced by two PKC activators. Inhibitors of the AC/cAMP/PKA pathway reversed both the SRIF- and GHRH-induced effects on GH release. Moreover, the GH release evoked by forskolin and 8-Br-cAMP were completely abolished by SRIF. The blockade of the NOS/NO pathway attenuated the GHRH-induced GH release but had minimal effects on the inhibitory actions of SRIF. In addition, inhibitors of the sGC/cGMP pathway did not modify the SRIF- or GHRH-induced regulation of GH release. Taken together, these findings indicate that the SRIF-induced inhibition of GH release is mediated by both the PLC/IP/PKC and the AC/cAMP/PKA pathways and not by the NOS/NO/sGC/cGMP pathway. In contrast, the GHRH-induced stimulation of GH secretion is mediated by both the AC/cAMP/PKA and the NOS/NO pathways and is independent of the sGC/cGMP pathway and the PLC/IP/PKC system.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP36589294	Arginine ethyl ester dihydrochloride		36589-29-4	Price

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