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Differential Subcellular Distribution of Tubulin Epitopes in Boar Spermatozoa: Recognition of Class III Beta-Tubulin Epitope in Sperm Tail

Differential Subcellular Distribution of Tubulin Epitopes in Boar Spermatozoa: Recognition of Class III Beta-Tubulin Epitope in Sperm Tail

J Pĕknicová, A Kubátová, V Sulimenko, E Dráberová, V Viklický, P Hozák, P Dráber

Biol Reprod. 2001 Sep;65(3):672-9.

PMID: 11514327

Abstract:

The exposure of tubulin epitopes was studied in ejaculated boar spermatozoa using a panel of four monoclonal antibodies specific to the N-terminal or C-terminal structural domains of tubulin and three monoclonal antibodies against class III beta-tubulin. The specificity of the antibodies was confirmed by immunoblotting. Immunocytochemical staining showed that antibodies discriminated between various parts of a spermatozoon, and that epitopes of class III beta-tubulin were present in the flagellum. A tubulin epitope from the C-terminal domain of beta-tubulin was detected in the triangular segment of the postacrosomal part of the sperm head. Its distribution changed after an A23187 ionophore-induced acrosome reaction, indicating that tubulin participates in the early stages of fertilization. Three monoclonal antibodies, TU-20, SDL.3D10, and TUJ1 directed against epitopes on the C-terminal end of neuron-specific class III beta-tubulin that is widely used as a neuronal marker, stained the flagella. The reactivity of TU-20 was further confirmed by absorbing the antibody with the immunizing peptide and by immunoelectron microscopy. Immunoblotting after two-dimensional electrophoresis revealed that the corresponding epitope was not present on all beta-tubulin isoforms. These results suggest that various tubulins are involved in the functional organization of the mammalian sperm flagellum and head.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP832115625	Neuronal Differentiation Inducer III - CAS 832115-62-5		832115-62-5	Price

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