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Direct Inhibitory Effect of Thyrotropin-Releasing Hormone on Isolated Cecal Circular Smooth Muscle Cells of Guinea Pig

Direct Inhibitory Effect of Thyrotropin-Releasing Hormone on Isolated Cecal Circular Smooth Muscle Cells of Guinea Pig

N Harada, Y Chijiiwa, H Akiho, H Okabe, H Nawata

Digestion. 1996;57(2):79-82.

PMID: 8786004

Abstract:

Smooth muscle cells isolated from cecal circular smooth muscle of the guinea pig were used to determine whether thyrotropin-releasing hormone (TRH) can inhibit the contractile response produced by 10(-6) M carbachol by exerting a direct action on muscle cells. In addition, the inhibitory effect of 2',5'-dideoxyadenosine (an inhibitor of adenylate cyclase), phorbol 12-myristate 13-acetate (an inhibitor of particulate guanylate cyclase), 6-anilinoquinoline-5,8-quinone (an inhibitor of nitric oxide synthase) on the TRH-induced relaxation of cecal circular smooth muscle cells was examined. TRH inhibited the contractile response produced by 10(-6) M carbachol in a concentration-dependent manner, with an IC50 value of 4 nM, 2',5'-Dideoxyadenosine and phorbol 12-myristate 13-acetate did not have any significant effect on the TRH-induced relaxation. On the other hand, 6-anilinoquinoline-5,8-quinone and N omega-nitro-L-arginine methyl ester significantly inhibited the relaxation produced by TRH. Our findings show that TRH has a direct inhibitory effect on the isolated cecal circular smooth muscle cells via activation of nitric oxide synthase and soluble guanylate cyclase.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP91300606	6-Anilinoquinoline-5,8-quinone		91300-60-6	Price

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