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Disassembly-driven Signal Turn-On Probes for Bimodal Detection of DNA With

Disassembly-driven Signal Turn-On Probes for Bimodal Detection of DNA With

Takashi Sakamoto, Daisaku Hasegawa, Kenzo Fujimoto

Org Biomol Chem. 2018 Oct 10;16(39):7157-7162.

PMID: 30246849

Abstract:

To image DNAs in vivo, molecular probes that can detect DNA with a highly transparent signal are required. In this study, we designed and synthesized amphiphilic molecular probes that can detect DNA with a highly transparent 19F NMR signal in a signal turn-on manner based on the self-assembly and target-dependent-disassembly of the probe molecules in aqueous solution. Fluorescence and dynamic light scattering studies revealed that these amphiphilic probes consisting of bisbenzimide H 33258 and 3,5-bis(trifluoromethyl)benzene are assembled and form aggregates in aqueous solution, and that the aggregates are collapsed when the probes bind to double-stranded DNA via selective interaction between the bisbenzimide H 33258 moiety and AATT double-stranded region of DNA. Since the target-dependent-disassembly of the probes caused turn-on of 19F NMR and fluorescence signals, bimodal detection of double-stranded DNA was successfully achieved.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP23491454-A	bisBenzimide H 33258		23491-45-4	Price

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