Home
Resources
Publications
Discovery of a Pyruvylated Peptide-Metabolizing Enzyme Using a Fluorescent Substrate-Based Protein Discovery Technique

Discovery of a Pyruvylated Peptide-Metabolizing Enzyme Using a Fluorescent Substrate-Based Protein Discovery Technique

Kentaro Yoshioka, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, Yasuteru Urano

Chem Commun (Camb). 2016 Mar 21;52(23):4377-80.

PMID: 26925595

Abstract:

We employed a fluorescent substrate-based target discovery approach to screen the enzymome for metabolic activity towards pyruvyl-amidated peptides, and identified an acylamino acid-releasing enzyme (APEH). Cells overexpressing APEH exhibited higher metabolic activity towards the probe, N-pyruvyl-leucyl-7-amido-4-methylcoumarin (Pyr-Leu-AMC), while the selective APEH inhibitor AA74-1 blocked the reaction. Metabolism of various pyruvylated peptides in liver lysate was almost completely blocked by AA74-1. Pyruvyl peptides are synthesized in response to oxidative stress, but their biological role is poorly understood; identification of a key contributor to their metabolism should stimulate research on pathways leading from oxidative stress to protein modification and biological output.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1361532004	AA74-1		1361532-00-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: