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Discrimination of Bovine Milk From Non-Dairy Milk by Lipids Fingerprinting Using Routine Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Discrimination of Bovine Milk From Non-Dairy Milk by Lipids Fingerprinting Using Routine Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Philippa England, Wenhao Tang, Markus Kostrzewa, Vahid Shahrezaei, Gerald Larrouy-Maumus

Sci Rep. 2020 Mar 20;10(1):5160.

PMID: 32198427

Abstract:

An important sustainable development goal for any country is to ensure food security by producing a sufficient and safe food supply. This is the case for bovine milk where addition of non-dairy milks such as vegetables (e.g., soya or coconut) has become a common source of adulteration and fraud. Conventionally, gas chromatography techniques are used to detect key lipids (e.g., triacylglycerols) has an effective read-out of assessing milks origins and to detect foreign milks in bovine milks. However, such approach requires several sample preparation steps and a dedicated laboratory environment, precluding a high throughput process. To cope with this need, here, we aimed to develop a novel and simple method without organic solvent extractions for the detection of bovine and non-dairy milks based on lipids fingerprint by routine MALDI-TOF mass spectrometry (MS). The optimized method relies on the simple dilution of milks in water followed by MALDI-TOF MS analyses in the positive linear ion mode and using a matrix consisting of a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid (super-DHB) solubilized at 10 mg/mL in 70% ethanol. This sensitive, inexpensive, and rapid method has potential for use in food authenticity applications.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP63542767	Super-DHB		63542-76-7	Price

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