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Dissecting the Functional Role of Polyketide Synthases in Dictyostelium Discoideum: Biosynthesis of the Differentiation Regulating Factor 4-methyl-5-pentylbenzene-1,3-diol

Dissecting the Functional Role of Polyketide Synthases in Dictyostelium Discoideum: Biosynthesis of the Differentiation Regulating Factor 4-methyl-5-pentylbenzene-1,3-diol

Ratna Ghosh, Arush Chhabra, Pallavi A Phatale, Subodh K Samrat, Jyoti Sharma, Anuradha Gosain, Debasisa Mohanty, Shweta Saran, Rajesh S Gokhale

J Biol Chem. 2008 Apr 25;283(17):11348-54.

PMID: 18252726

Abstract:

Dictyostelium discoideum exhibits the largest repository of polyketide synthase (PKS) proteins of all known genomes. However, the functional relevance of these proteins in the biology of this organism remains largely obscure. On the basis of computational, biochemical, and gene expression studies, we propose that the multifunctional Dictyostelium PKS (DiPKS) protein DiPKS1 could be involved in the biosynthesis of the differentiation regulating factor 4-methyl-5-pentylbenzene-1,3-diol (MPBD). Our cell-free reconstitution studies of a novel acyl carrier protein Type III PKS didomain from DiPKS1 revealed a crucial role of protein-protein interactions in determining the final biosynthetic product. Whereas the Type III PKS domain by itself primarily produces acyl pyrones, the presence of the interacting acyl carrier protein domain modulates the catalytic activity to produce the alkyl resorcinol scaffold of MPBD. Furthermore, we have characterized an O-methyltransferase (OMT12) from Dictyostelium with the capability to modify this resorcinol ring to synthesize a variant of MPBD. We propose that such a modification in vivo could in fact provide subtle variations in biological function and specificity. In addition, we have performed systematic computational analysis of 45 multidomain PKSs, which revealed several unique features in DiPKS proteins. Our studies provide a new perspective in understanding mechanisms by which metabolic diversity could be generated by combining existing functional scaffolds.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP538681	Pentylbenzene		538-68-1	Price

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