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Distinct Isoforms of Protein Kinase C Are Involved in Human Eosinophil Functions Induced by Platelet-Activating Factor

Distinct Isoforms of Protein Kinase C Are Involved in Human Eosinophil Functions Induced by Platelet-Activating Factor

Takumi Takizawa, Masahiko Kato, Masato Suzuki, Atsushi Tachibana, Youichi Motegi, Toru Fujiu, Hirokazu Kimura, Hirokazu Arakawa, Hiroyuki Mochizuki, Kenichi Tokuyama, Akihiro Morikawa

Int Arch Allergy Immunol. 2003 Jun;131 Suppl 1:15-9.

PMID: 12771544

Abstract:

Background:
Platelet-activating factor (PAF) is a potent stimulator of eosinophils. Recently, treatment with a protein kinase C (PKC) inhibitor which generally inhibits PKC isoforms has been shown to modulate several eosinophil functions in distinct manners, in that PKC inhibition enhanced CD11b expression and cellular adhesion, but inhibited superoxide generation and degranulation in PAF-stimulated human eosinophils. These results suggested that distinct PKC isoforms were likely to be involved in each eosinophil function induced by PAF. We have therefore investigated whether or not the PKC isoforms involved in PAF-induced CD11b expression and superoxide generation were different.
Methods:
Human eosinophils prepared from healthy volunteers were treated with PKC inhibitors, bis-indolylmaleimide I (BisI; a general PKC inhibitor), myristoylated PKC inhibitor peptide (myr-psiPKC; a PKCalpha, beta and delta inhibitor) and rottlerin (a PKCdelta inhibitor), followed by stimulation with PAF. CD11b expression was determined using flow cytometry and superoxide generation was evaluated using a cytochrome c reduction assay.
Results:
BisI treatment led to enhancement of PAF-induced CD11b expression, while myr-psiPKC and rottlerin did not. In contrast, PAF-induced superoxide generation was inhibited by treatment with BisI, myr-psiPKC and rottlerin.
Conclusions:
PKCalpha, beta and delta are not involved in PAF-induced CD11b expression, but PKCdelta is involved in the PAF-induced activation of superoxide anion generation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4244480	Protein Kinase C Inhibitor, Myristoylated			Price

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