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Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe 3 O 4-GO): A new strategy for covalent enzyme (lipase) immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD

Mohammad Heidarizadeh, Esmail Doustkhah, Sadegh Rostamnia, Parisa Fathi Rezaei, Farshad Darvishi Harzevili, Behzad Zeynizadeh

Int J Biol Macromol. 2017 Aug;101:696-702.

PMID: 28363653

Abstract:

Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4/graphene oxide (GO) via a post-modification. This post modification was achieved in alternation to glutaraldehyde post-modification. The activity of immobilized lipase had not a significant loss in the activity while on the other hand, it is simply extractable (by keeping its major activity) from reaction crude by a magnet. Each step of immobilization was carefully monitored by characterization and all were successfully proved. SEM, TEM, XRD, EDX, and FTIR were used to characterize the support and immobilization process.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP1956112 4-Nitrophenyl dodecanoate 4-Nitrophenyl dodecanoate 1956-11-2 Price
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