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Double-sandwich Enzyme-Linked Immunosorbent Assay for Determination of Escherichia Coli Heat-Labile Porcine Enterotoxins

Double-sandwich Enzyme-Linked Immunosorbent Assay for Determination of Escherichia Coli Heat-Labile Porcine Enterotoxins

B Picard, J M Alessandri, Y Duval-Iflah

Vet Microbiol. 1988 May;17(1):83-90.

PMID: 3051649

Abstract:

A "double-sandwich" ELISA for the detection and measurement of a heat-labile enterotoxin produced by porcine enterotoxigenic strains of Escherichia coli (LTp) is described. In contrast with other heat-labile toxins, LTp did not bind to agarose gels and exhibited a very low affinity for GM1 in the classical GM1-ELISA technique. The similarity of LTp with cholera toxin was confirmed by immunoblotting. This property allowed the binding of LTp to rabbit IgG anti-cholera toxin antibodies (covalently linked to polystyrene plates) and sheep anti-cholera toxin serum. The immunocomplex was revealed by anti-sheep immunoglobulin antibodies conjugated with peroxidase. Application of the "double-sandwich" ELISA to the quantitation of toxin production by two strains, which differ only in the presence or the absence of the K88ab antigen, showed that the Ent+, K88+ strain produced significantly less toxin than the Ent+, K88- derivative.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414504	Anti-Cholera Toxin antibody produced in rabbit			Price

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