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Down-regulation of Increased TRAF6 Expression in the Peripheral Mononuclear Cells of Patients With Primary Sjögren's Syndrome by an EBV-EBER1-specific Synthetic Single-Stranded Complementary DNA Molecule

Down-regulation of Increased TRAF6 Expression in the Peripheral Mononuclear Cells of Patients With Primary Sjögren's Syndrome by an EBV-EBER1-specific Synthetic Single-Stranded Complementary DNA Molecule

Sándor Sipka, Erika Zilahi, Gábor Papp, Ji-Qing Chen, Andrea Nagy, Katalin Hegyi, József Kónya, Margit Zeher

Int J Rheum Dis. 2017 May;20(5):614-621.

PMID: 28470929

Abstract:

Aim:
We described earlier a simultaneously increased that the increased expression of miRNA-146a/b was accompanied by an increase in the expression of and TRAF6 and a decrease in the expression of IRAK1 genes in the peripheral mononuclear cells (PBMCs) of patients with primary Sjogren's syndrome (pSS) patients. Recently, the expression of EBV encoded. RNA (EBER) was published in the B cells of salivary glands of in pSS. In the present study, we applied an EBV-EBER1 specific synthetic single stranded complementary DNA molecule (EBV-EBER1-cDNA) to test whether any EBER1 related effect exists also in PBMCs of pSS patients.
Methods:
In the PBMCs of pSS patients and healthy controls, we investigated in vitro the effects of a synthetic single stranded EBV-EBER1-cDNA molecule, synthetic double-stranded (ds)RNA polyinosinic-polycytidylic acid [poly (I:C)] and polyadenylic acid potassium salt poly-adenylic acid [poly-(A)] on the expression of TRAF6 gene tested by qRTPCR. The release of interferon -α was detected by ELISA.
Results:
EBV-EBER1-cDNA resulted in a significant reduction in the expression of TRAF6 in the cells of patients, but in the healthy controls not, whereas the treatments with poly (I:C) and poly-(A) could not reduce the TRAF6 over-expression. No release of EBER1 could be observed in the culture supernatants of patients with pSS. Only the treatment with poly (I:C) resulted in a significant increase of interferon -α release, and only in the heathy controls. No release of EBER1 molecules took place during the culturing of cells. EBV-EBER- cDNA acted functionally on the cells of patients only.
Conclusion:
These findings give a further evidence of the linkage between EBV and pSS, furthermore, they show the possible role of EBV-EBER1 in the induction of increased TRAF6 expression in the peripheral B cells of Sjögren's patients.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP26936414	Polyinosinic acid potassium salt		26936-41-4	Price
AP31852296	Polyinosinic-polycytidylic acid potassium salt		31852-29-6	Price

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