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Dual Function of Ammonium Acetate in Acetone-Butanol-Ethanol Fermentation by Clostridium Acetobutylicum

Dual Function of Ammonium Acetate in Acetone-Butanol-Ethanol Fermentation by Clostridium Acetobutylicum

Shubo Li, Yuan Zhou, Zhiting Luo, Yanyan Cui, Yu Xu, Lihua Lin, Mouming Zhao, Yuan Guo, Zongwen Pang

Bioresour Technol. 2018 Nov;267:319-325.

PMID: 30029177

Abstract:

In this study, a compound nitrogen source, integrating the advantages of ammonium acetate and soybean meal, was proposed to further improve acetone-butanol-ethanol fermentation. Unfortunately, this compound nitrogen source was found to effectively inhibit cellular performance, as the introduction of NH4+ significantly decreased the yield of butanol and total solvents by 34.78% and 35.14%, to only 6.62 g/L and 10.76 g/L, respectively. Meanwhile, the regulatory mechanism was further elucidated at different levels. As a result, the NH4+ could down-regulate the transcriptional levels of key genes involved in butanol synthesis, and the activity of acetoacetyl-CoA/acyl-CoA transferase, and then decrease the accumulation of key intermediates. Therefore, ammonium acetate has a dual function in ABE fermentation, as it effectively improves ABE fermentation when it is the sole nitrogen source but significantly decreases fermentation performance in the presence of soybean meal, broadening the understanding of nitrogen regulation mechanism of C. acetobutylicum.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP631618	Ammonium acetate		631-61-8	Price

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