Home
Resources
Publications
Duplication of Single Stranded DNA Catalyzed by Calf Thymus DNA Polymerase Alpha

Duplication of Single Stranded DNA Catalyzed by Calf Thymus DNA Polymerase Alpha

E Wang, D Henner, J J Furth

Nucleic Acids Res. 1976 Jan;3(1):129-47.

PMID: 175352

Abstract:

Purified calf thymus DNA polymerase alpha is inactive with native DNA as template and shows little activity with denatured DNA. DNA synthesis with denatured DNA as template is greatly stimulated by the addition of a nuclease which initially copurifies with DNA polymerase but is separated from the polymerase on DEAE-cellulose chromatography. A limit digest of nuclease treated native DNA which is then denatured is replicated 80-95%; extensive replication is also obtained with native DNA partially degraded by pancreatic DNase and then denatured. The product of the reaction with calf thymus nuclease-treated DNA as template is double-stranded DNA with a hairpin (looped back) structure.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411064	Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: