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Effect of 2-deoxy-D-glucose on Gellan Gum Biosynthesis by Sphingomonas Paucimobilis

Effect of 2-deoxy-D-glucose on Gellan Gum Biosynthesis by Sphingomonas Paucimobilis

Guilan Zhu, Na Guo, Yanan Yong, Yawen Xiong, Qunyi Tong

Bioprocess Biosyst Eng. 2019 May;42(5):897-900.

PMID: 30671627

Abstract:

2-Deoxy-D-glucose (2-DG) is a non-metabolizable glucose analogue and competitive inhibitor of glycolysis. Effect of 2-DG on gellan gum biosynthesis by Sphingomonas paucimobilis ATCC31461 were studied in this research. The concentration and the addition time of 2-DG significantly affected the biomass and gellan gum accumulation. The maximum gellan gum yield of 20.78 g/L was obtained with the addition of 50 µg/L of 2-DG at 24 h. The mechanism of 2-DG addition favoring to gellan production was revealed by determining the activities of key enzymes. Results indicated that 2-DG addition increased the activities of glucosyltransferase and inhibited UDP-glucose pyrophosphorylase activity. The result indicated that 2-DG inhibited glycolysis and changed metabolic driving force to activate gellan gum biosynthesis metabolism pathways.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP154176-A	2-Deoxy-D-glucose		154-17-6	Price

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