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Effect of a 2-hydroxylated Fatty Acid on Cholesterol-Rich Membrane Domains

Effect of a 2-hydroxylated Fatty Acid on Cholesterol-Rich Membrane Domains

Jesús Prades, Sérgio S Funari, Manuel Gomez-Florit, Oliver Vögler, Francisca Barceló

Mol Membr Biol. 2012 Dec;29(8):333-43.

PMID: 22830943

Abstract:

2-Hydroxyoleic acid (2OHOA) is a synthetic fatty acid with antihypertensive properties that is able to alter structural membranes properties. The main purpose of this study was to analyze the effect of 2OHOA on the membrane architecture in cholesterol (Cho)-rich domains. For this purpose, model membranes mimicking the composition of lipid rafts and PC- or PE-Cho-rich domains were examined in the absence and presence of 2OHOA by synchrotron X-ray diffraction, atomic force microscopy (AFM) and microcalorimetry (DSC) techniques. Our results demonstrate that 2OHOA phase separates from lipid raft domains and affects the lateral organization of lipids in the membrane. In model raft membranes, 2OHOA interacted with the sphingomyelin (SM) gel phase increasing the thickness of the water layer, which should lead to increased bilayer fluidity. The hydrogen binding competition between 2OHOA and Cho could favour the enrichment of 2OHOA in SM domains separated from the SM-Cho domains, resulting in an enhanced phase separation into SM-2OHOA-rich liquid-disordered (non-raft) and SM-Cho-rich liquid-ordered (raft) domains. The segregation into 2OHOA-rich/Cho-poor and 2OHOA-poor/Cho-rich domains was also observed in PC bilayers.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP56472298	2OHOA		56472-29-8	Price

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