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Effect of A(2A) Receptor Antagonist (SCH 442416) on the mRNA Expression of Glutamate Aspartate Transporter and Glutamine Synthetase in Rat Retinal Müller Cells Under Hypoxic Conditions in Vitro

Effect of A(2A) Receptor Antagonist (SCH 442416) on the mRNA Expression of Glutamate Aspartate Transporter and Glutamine Synthetase in Rat Retinal Müller Cells Under Hypoxic Conditions in Vitro

Jun Yu, Xin Huang, Qirong Wu, Jun Wang, Xiaoyan Yu, Peiquan Zhao

Exp Ther Med. 2012 May;3(5):803-806.

PMID: 22969972

Abstract:

The purpose of the present study was to investigate the effect of the A(2A) receptor antagonist (SCH 442416) on the mRNA expression of glutamate aspartate transporter (GLAST) and glutamine synthetase (GS) in rat retinal Müller cells under hypoxic conditions in vitro. Immunofluorescent staining of GS and GFAP was used for the identification of Müller cells. The GLAST and GS mRNA expression of Müller cells treated with 0.1, 1 and 10 μM SCH 442416 under hypoxic conditions was examined by real-time PCR. Müller cells increased the mRNA expression of GLAST under hypoxic conditions; those treated with 0.1 μM SCH 442416 showed a further significant increase in the mRNA expression of GLAST in vitro. Although the mRNA expression of GS was decreased under hypoxic conditions, the mRNA expression was increased when Müller cells were treated with 0.1 μM SCH 442416. A(2A) receptor antagonist increased the GLAST and GS expression of Müller cells and accelerated the clearance of extracellular glutamate under hypoxic conditions in vitro.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP316173576	SCH-442416		316173-57-6	Price

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