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Effect of Acetohydroxamic Acid on Rumen Urease Activity in Vitro

Effect of Acetohydroxamic Acid on Rumen Urease Activity in Vitro

H P Makkar, O P Sharma, R K Dawra, S S Negi

J Dairy Sci. 1981 Apr;64(4):643-8.

PMID: 7264024

Abstract:

Acetohydroxamic acid at a concentration of 1 X 10(-6) M, 6 X 10(-5) M, and 1 X 10(-3) M inhibited urease of intact rumen microbes in vitro by 11%, 50%, and 74%. Inhibition of rumen urease by the acid reached equilibrium state, unlike jack bean urease. Inhibition was maximum over a broad range of pH (8 to 10) and it did not resemble the pH activity profile of rumen urease. Sulfhydryl compounds did not reverse the inhibition; however, addition of these compounds prior to acetohydroxamic acid addition prevented inhibition. The nature of inhibition was noncompetitive with inhibitor constant 4.8 X 10(-5) M. Acetohydroxamic acid at a concentration that produced 50% urease inhibition did not affect rumen cellulase and proteolytic enzymes in vitro. The complex of acetohydroxamic acid-rumen urease is dissociable on dialysis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP546883	Acetohydroxamic acid		546-88-3	Price

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