Home
Resources
Publications
Effect of Angiotensin II, ATP, and Ionophore A23187 on Potassium Efflux in Adrenal Glomerulosa Cells

Effect of Angiotensin II, ATP, and Ionophore A23187 on Potassium Efflux in Adrenal Glomerulosa Cells

M V Lobo, E T Marusic

Am J Physiol. 1986 Feb;250(2 Pt 1):E125-30.

PMID: 3082214

Abstract:

Angiotensin II stimulus on perifused bovine adrenal glomerulosa cells elicited an increase in 86Rb efflux from cells previously equilibrated with the radioisotope. When 45Ca fluxes were measured under similar conditions, it was observed that Ca and Rb effluxes occurred within the first 30 s of the addition of the hormone and were independent of the presence of external Ca. The 86Rb efflux due to angiotensin II was inhibited by quinine and apamin. The hypothesis that the angiotensin II response is a consequence of an increase in the K permeability of the glomerulosa cell membrane triggered by an increase in cytosolic Ca is supported by the finding that the divalent cation ionophore A23187 also initiated 86Rb or K loss (as measured by an external K electrode). This increased K conductance was also seen with 10(-4) M ATP. Quinine and apamin greatly reduced the effect of ATP or A23187 on 86Rb or K release in adrenal glomerulosa cells. The results suggest that Ca-dependent K channels or carriers are present in the membranes of bovine adrenal glomerulosa cells and are sensitive to hormonal stimulus.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP69271983	Potassium ionophore II		69271-98-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: