Home
Resources
Publications
Effect of Norcantharidin on the Proliferation, Apoptosis, and Cell Cycle of Human Mesangial Cells

Effect of Norcantharidin on the Proliferation, Apoptosis, and Cell Cycle of Human Mesangial Cells

Kun Ye, Qiaoyu Wei, Zhifeng Gong, Yunfeng Huang, Hong Liu, Ying Li, Xiaomei Peng

Ren Fail. 2017 Nov;39(1):458-464.

PMID: 28393592

Abstract:

Aims:
Norcantharidin (NCTD) regulates immune system function and reduces proteinuria. We sought to investigate the effect of NCTD on proliferation, apoptosis and cell cycle of cultured human mesangial cells (HMC) in vitro.
Methods:
HMC cells were divided into a normal control group, and various concentrations of NCTD group (2.5, 5, 10, 20, or 40 μg/mL). Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, apoptosis was detected by Annexin V/propidium iodide (PI) assays, and morphological analysis was performed by Hoechest 33258 staining. Finally, cell cycle was analyzed by flow cytometry.
Results:
NCTD dose and time dependently inhibits HMC proliferation significantly (p < .05). Apoptosis dose and time dependently increased after NCTD treatment. Cell-cycle analysis revealed that the number of cells in the G2 phase increased significantly, whereas the fraction of cells in the S phase decreased, especially 24 h after 5 μg/ml NCTD treatment.
Conclusion:
NCTD inhibits HMC cell proliferation, induces apoptosis, and affects the cell cycle.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP29745048	Norcantharidin		29745-04-8	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: