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Effect of Perfluorooctane Sulfonate on the Conformation of Wheat Germ Acid Phosphatase

Effect of Perfluorooctane Sulfonate on the Conformation of Wheat Germ Acid Phosphatase

Dongmei Xu, Jianchang Jin, Tong Shen, Yanhua Wang

Bull Environ Contam Toxicol. 2013 Nov;91(5):577-82.

PMID: 24068464

Abstract:

Fluorescence spectroscopy was used to study the quenching mechanism, the type of force and the binding sites of perfluorooctane sulfonate (PFOS) on wheat germ acid phosphatase (ACPase). The results showed that the quenching effect of PFOS on ACPase was mainly due to a static quenching mechanism that occurred via the formation of hydrogen bonds and van der Waals forces. The results from synchronous fluorescence spectroscopy demonstrated that PFOS interacts with ACPase close to the tryptophan residues. In addition, synchronous fluorescence spectroscopy also showed that PFOS increases the hydrophobicity of the microenvironment of the tyrosine residues, hence decreasing the local polarity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001778-B	Phosphatase, Acid from wheat germ		9001-77-8	Price

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