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Effect of Phosphatidylcholine, Phosphatidylethanolamine and Lysophosphatidylcholine on the Protein C/protein S Anticoagulation System

Effect of Phosphatidylcholine, Phosphatidylethanolamine and Lysophosphatidylcholine on the Protein C/protein S Anticoagulation System

Tomohide Tsuda, Hajime Yoshimura, Naotaka Hamasaki

Blood Coagul Fibrinolysis. 2006 Sep;17(6):453-8.

PMID: 16905948

Abstract:

Phosphatidylserine is known to significantly accelerate the blood coagulation reaction. In a previous communication submitted for publication, we demonstrated that phosphatidylcholine, phosphatidylethanolamine and lysophosphatidylcholine showed effects on the blood coagulation reaction using the factor Xa-prothrombin reaction system, and discuss a new function of membrane phospholipids. The present study examined the role of phospholipids in the blood coagulation regulatory reaction (anticoagulation system), by studying the effects of phospholipids on the protein C/protein S reaction. We have established quantitative methods for measuring activated protein C activity and protein S activity, and used them to measure their activity after the addition of liposomes with different phospholipid compositions. We found that phosphatidylcholine inhibited activated protein C and protein S activities in a dose-dependent manner, as in the factor Xa-prothrombin reaction system. On the other hand, phosphatidylethanolamine and lysophosphatidylcholine showed no effect on activated protein C activity. Phosphatidylethanolamine inhibited and lysophosphatidylcholine accelerated coagulation activity in the factor Xa-prothrombin system, but such effects were not observed in the protein C/protein S reaction system. The coagulation and anticoagulation reactions are exquisitely balanced by thrombin, with a role both as a procoagulant and anticoagulant. Therefore, it is understandable that phosphatidylethanolamine and lysophosphatidylcholine show different effects in the factor Xa-prothrombin and protein C/protein S reaction systems. It appears that coagulation and anticoagulation reactions are co-ordinated and controlled by changes in phospholipid composition of the cellular membrane where the coagulation reaction takes place.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP39382086-A	Phosphatidylethanolamine		39382-08-6	Price

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