Home
Resources
Publications
[Effect of PKF118-310 on Cell Cycle and Proliferation of K562 Cells and Its Mechanism]

[Effect of PKF118-310 on Cell Cycle and Proliferation of K562 Cells and Its Mechanism]

Dan-Yu Wang, Wei-Ming Li, Meng-Di Jing, Hai-Yan Cui, Bo Lu, Xin Wang, Ze-Lin Liu

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Aug;23(4):989-93.

PMID: 26314431

Abstract:

Objetive: To investigate the effects of PKF118-310 on cell cycle and proliferation of K562 cell lines and its mechanism.
Methods:
After treatment of PKF118-310 with different concentration, the proliferation inhibition on K562 cell lines was detected by MTT, the existance of β-catenin and TCF-4 in the cells was observed by immunohistochemistry. The change of the cell cycle was detected by flow cytometry. The expressions of caspase-3, β-catenin, TCF and BCL-9 were detected by Western blot.
Results:
PKF118-310 can inhibit the proliferation of K562 cell line by S phase blocking. The β-catenin and TCF in the cells were observed by immunohistochemistry. After treating this cell line with PKF118-310 of different concentrations for 72 h, the expression level of caspase-3 increased, the expression levels of β-catenin, TCF and BCL-9 significantly decreased.
Conclusion:
PKF118-310 induces cycle arest of K562 cells at the S phase and inhibits the proliferation of these cells through decreasing β-catenin/TCF/BCL-9 thrascriptional activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP84822	PKF118-310		84-82-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: