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Effect of Proteasome Inhibitor Clasto-Lactacystin-Beta-Lactone on the Proteome of the Haloarchaeon Haloferax Volcanii

Effect of Proteasome Inhibitor Clasto-Lactacystin-Beta-Lactone on the Proteome of the Haloarchaeon Haloferax Volcanii

P Aaron Kirkland, Christopher J Reuter, Julie A Maupin-Furlow

Microbiology. 2007 Jul;153(Pt 7):2271-2280.

PMID: 17600071

Abstract:

Proteasomes play key roles in a variety of eukaryotic cell functions, including translation, transcription, metabolism, DNA repair and cell-cycle control. The biological functions of these multicatalytic proteases in archaea, however, are poorly understood. In this study, Haloferax volcanii was used as a model to determine the influence the proteasome-specific inhibitor clasto-lactacystin-beta-lactone (cLbetaL) has on archaeal proteome composition. Addition of 20-30 microM cLbetaL had a widespread effect on the proteome, with a 38-42 % increase in the number of 2-D gel electrophoresis (2-DE) protein spots, from an average of 627 to 1036 spots. Protein identities for 17 of the spots that were easily separated by 2-DE and unique and/or increased 2- to 14-fold in the cLbetaL-treated cells were determined by tandem mass spectrometry (MS/MS). These included protein homologues of the DJ-1/ThiJ family, mobilization of sulfur system, translation elongation factor EF-1 A, ribosomal proteins, tubulin-like FtsZ, divalent metal ABC transporter, dihydroxyacetone kinase DhaL, aldehyde dehydrogenase and 2-oxoacid decarboxylase E1beta. Based on these results, inhibition of H. volcanii proteasomes had a global influence on proteome composition, including proteins involved in central functions of the cell.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP154226605-A	clasto-Lactacystin β-lactone		154226-60-5	Price

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