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[Effect of TNF-α on the Ability of Stem Cells From Human Exfoliated Deciduous Teeth to Promote Osteoclastogenesis]

[Effect of TNF-α on the Ability of Stem Cells From Human Exfoliated Deciduous Teeth to Promote Osteoclastogenesis]

Yu-Chen Wang, Chen Wang, Na Liu, Chuan Cai, Wei Liu, Lu-Lu Xu

Shanghai Kou Qiang Yi Xue. 2018 Oct;27(5):449-454.

PMID: 30680384

Abstract:

Purpose:
To investigate the effect of tumor necrosis factor-α (TNF-α) on the ability of stem cells from human exfoliated deciduous teeth (SHED) to promote osteoclastogenesis.
Methods:
SHED were obtained from deciduous teeth and isolated, purified, cultured in vitro. An indirect co-culture system of SHED and osteoclast precursor peripheral blood mononuclear cells (PBMCs) was established. The expression of osteoclastic gene from PBMCs and NF-κB from SHED were determined after treatment with TNF-α (0, 5, 10, 50, 100 ng/mL) by real-time RT-PCR and Western blot. SPSS 19.0 software package was used for statistical analysis.
Results:
Under the stimulation of 10ng/mL TNF-α, the expression of CTSK and TRAP was markedly upregulated in PBMCs. Meanwhile, the results of Western blot and real-time RT-PCR showed that the expression of cytoplasmic phosphorylated inhibitor of NF-κB α (p-IκBα) and nuclear p65 in SHED were significantly higher than that without TNF-α stimulation after 10 ng/mL TNF-α treatment.
Conclusions:
TNF-α regulates the ability of SHED to promote osteoclastogenesis through NF-κB signal pathways.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248889	TNF-α human			Price

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