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Effect of Zinc Supplements in the Attenuated Cardioprotective Effect of Ischemic Preconditioning in Hyperlipidemic Rat Heart

Effect of Zinc Supplements in the Attenuated Cardioprotective Effect of Ischemic Preconditioning in Hyperlipidemic Rat Heart

Sunil Kumar Kansal, Uma Jyoti, Samridhi Sharma, Arun Kaura, Rahul Deshmukh, Sandeep Goyal

Naunyn Schmiedebergs Arch Pharmacol. 2015 Jun;388(6):635-41.

PMID: 25743572

Abstract:

Hyperlipidemia is regarded as independent risk factor in the development of ischemic heart disease, and it can increase the myocardial susceptibility to ischemia-/reperfusion (I/R)-induced injury. Hyperlipidemia attenuates the cardioprotective response of ischemic preconditioning (IPC). The present study investigated the effect of zinc supplements in the attenuated cardioprotective effect of ischemic preconditioning in hyperlipidemic rat hearts. Hyperlipidemia was induced in rat by feeding high-fat diet (HFD) for 6 weeks then the serum lipid profile was observed. In experiment, the isolated Langendorff rat heart preparation was subjected to 4 cycles of ischemic preconditioning (IPC), then 30 min of ischemia followed by 120 min of reperfusion. Myocardial infarct size was elaborated morphologically by triphenyltetrazolium chloride (TTC) staining and biochemically by lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) release from coronary effluent and left ventricular collagen content. However, the effect of zinc supplement, i.e., zinc pyrithione (10 μM) perfused during reperfusion for 120 min, significantly abrogated the attenuated cardioprotective effect of ischemic preconditioning in hyperlipidemic rat heart whereas administration of chelator of this zinc ionophore, i.e., N,N,N',N'-tetrakis(2-pyridylmethyl)ethylene diamine (TPEN; 10 μM), perfused during reperfusion 2 min before the perfusion of zinc pyrithione abrogated the cardioprotective effect of zinc supplement during experiment in hyperlipidemic rat heart. Thus, the administration of zinc supplements limits the infarct size, LDH, and CK-MB and enhanced the collagen level which suggests that the attenuated cardioprotective effect of IPC in hyperlipidemic rat is due to zinc loss during reperfusion caused by ischemia/reperfusion.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1634022	Zinc ionophore I		1634-02-2	Price
AP32195554-A	Chloride ionophore I		32195-55-4	Price

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