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Effects of Adenosine 3',5'-cyclic Monophosphate, Forskolin, and Cholera Toxin on Hormone Production in Human Term Placental Cells

Effects of Adenosine 3',5'-cyclic Monophosphate, Forskolin, and Cholera Toxin on Hormone Production in Human Term Placental Cells

F Zhou, B H Yuen, P C Leung

Am J Obstet Gynecol. 1987 Feb;156(2):420-4.

PMID: 3030108

Abstract:

Trophoblast cells from human term placenta in monolayer culture were used to investigate the role of adenosine 3',5'-cyclic monophosphate in the production of human chorionic gonadotropin and estradiol-17 beta. The intracellular concentration of adenosine 3',5'-cyclic monophosphate was elevated by (1) addition of an adenosine 3',5'-cyclic monophosphate analogue, 8-bromoadenosine 3',5'-cyclic monophosphate, (2) inhibition of the hydrolysis of Gs-GTP complex by cholera toxin, and (3) direct stimulation of adenylate cyclase with forskolin. Addition of 2 mmol/L of 8-bromoadenosine 3',5'-cyclic monophosphate markedly increased the accumulation of human chorionic gonadotropin in the culture medium on days 2, 3, and 4 of treatment. Likewise, addition of cholera toxin (0.2 microgram/ml) or forskolin (50 mumol/L) also enhanced human chorionic gonadotropin production. On the other hand, the production of estradiol-17 beta was significantly inhibited by 8-bromoadenosine 3',5'-cyclic monophosphate, at the same time that human chorionic gonadotropin production was enhanced in the same experiments. These results further support a differential role of adenosine 3',5'-cyclic monophosphate on human chorionic gonadotropin and estradiol-17 beta production in the human term placenta.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP60924	Adenosine 3′,5′-cyclic monophosphate		60-92-4	Price

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