Home
Resources
Publications
Effects of ceranib-2 on Cell Survival and TNF-alpha in Colon Cancer Cell Line

Effects of ceranib-2 on Cell Survival and TNF-alpha in Colon Cancer Cell Line

M Baspinar, R Ozyurt, G Kus, O Kutlay, M Ozkurt, N Erkasap, S Kabadere, N F Yasar, S Erkasap

Bratisl Lek Listy. 2017;118(7):391-393.

PMID: 28766347

Abstract:

Objective:
The aim of this study was to investigate the effects of a novel anti-cancer drug, ceranib-2, which targets the acid ceramidase, in human colon cancer cell line.
Materials and methods:
The cell lines were treated with 50 µM of ceranib-2. Relative mRNA expression of TNF-alpha, TNF-R1 and ASAH were assessed by quantitative RT-PCR.
Results:
Ceranib-2 reduced cell viability in a dose-dependent manner and the apoptotic values of cells following treatment with the dose of 50 µM were reduced significantly both at 24 h and 48 h compared to the control cells (p < 0.001). TNF-alpha receptor 1 (TNF-R1) mRNA levels were reduced significantly in the cell lines treated with both 25 µM and 50 µM of ceranib-2 for 24 h compared to the control cells (p < 0.05), whereas the difference between the treatment and the control cell lines diminished at 48 h. The human acid ceramidase gene (ASAH) mRNA levels were significantly higher in the cell lines treated with 50 µM of ceranib-2 for 48 h than in the other cell lines (p < 0.001).
Conclusion:
The study shows that ceranib-2 increased apoptosis by inducing ASAH expression and reduced TNF-R1 expression in human colon cancer cell lines in a dose and time-dependent manner (Fig. 3, Ref. 17).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1402830754	Ceranib-2		1402830-75-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: