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Effects of Dexamethasone and Ibuprofen on LPS-induced Gene Expression of TNF Alpha, IL-1 Beta, and MIP-1 Alpha in Rat Lung

Effects of Dexamethasone and Ibuprofen on LPS-induced Gene Expression of TNF Alpha, IL-1 Beta, and MIP-1 Alpha in Rat Lung

H B Qiu, J Q Pan, Y Q Zhao, D C Chen

Zhongguo Yao Li Xue Bao. 1997 Mar;18(2):165-8.

PMID: 10072973

Abstract:

Aim:
To study the kinetics of tumor necrosis factor alpha (TNF alpha), interleukine-1 (IL-1 beta), and macrophage inflammatory protein-1 alpha (MIP-1 alpha) gene expression in rat lung after i.p. lipopolysaccharides (LPS) and the effect of dexamethasone (Dex) and ibuprofen (Ibu) on the cytokines gene expression.
Methods:
The amount of Evans blue in lung was measured by fluorescence method. The mRNA levels of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung were assessed by slot blot analysis.
Results:
The mRNA levels of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung after i.p. LPS increased in a dose-dependent manner, and peaked at 2, 6, and 12 h, respectively. Both Dex 50 mg.kg-1 and Ibu 90 mg.kg-1 injected at 1 h before i.p. LPS markedly decreased the content of Evans blue in lung at 1 h after i.p. LPS. After Dex or Ibu pretreatment, the peak levels of TNF alpha, IL-1 beta, and MIP-1 alpha mRNA decreased markedly compared with LPS alone.
Conclusion:
The gene expression of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung increased after i.p. LPS. Dex and Ibu prevented LPS-induced lung injury through inhibiting the cytokines gene expression.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413118	MIP-1 beta from rat			Price

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