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[Effects of Garcinia Acid Combined With Daunorubicin on Expression of Pregnane X Receptor in Leukemia Cell Line K562/A02]

[Effects of Garcinia Acid Combined With Daunorubicin on Expression of Pregnane X Receptor in Leukemia Cell Line K562/A02]

Han Zhu, Bao-An Chen, Xiao-Hui Cai, Fei Wang, Chong Gao, Jian Cheng, Xiao-Ping Zhang, Lu Dai, Guo-Hua Xia, Rong Fu, Qing-Long Guo

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Aug;23(4):956-61.

PMID: 26314425

Abstract:

Objective:
To explore the expression of PXR (Pregnane X receptor) in several malignant hematological cell lines, and to investigate the reversal effect of Gambogic acid (GA) on multi-drug resistance (MDR) of K562/A02 cell line and its reversal mechanism.
Methods:
Transcription of PXR was detected by real-time PCR in several malignant hematological cell lines. The growth inhibition rate of K562/A02 in different experimental groups was assayed by MTT method, and the expression of PXR protein was measured by Western blot.
Results:
PXR gene transcription could be detected in several hematological malignancy cell lines, and it was significantly higher in K562/A02 cell line, compared with the other cell lines used in this experiment. Low-dose GA could enhance cell growth inhibition rate, increasing the effect of chemotherapy, which may be associated with down-regulation of PXR expression. PXR gene transcription and protein expression in GA and DNR+GA groups decreased as compared with control group and the DNR group, suggesting that low-dose GA can down-regulate PXR gene transcription and protein expression.
Conclusion:
PXR gene transcription can be detected in several hematological malignancy cell line, which is significantly higher in K562/A02 cell line, as compared with the other cell lines used in this experiment. Low-dose GA can enhance cell growth inhibition rate, increasing the effect of chemotherapy, which may be associated with down-regulation of PXR expression.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP27750136	(+)-Garcinia acid		27750-13-6	Price

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