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Effects of Glycerol on Enzymatic Hydrolysis and Ethanol Production Using Sugarcane Bagasse Pretreated by Acidified Glycerol Solution

Effects of Glycerol on Enzymatic Hydrolysis and Ethanol Production Using Sugarcane Bagasse Pretreated by Acidified Glycerol Solution

Zhanying Zhang, Heng H Wong, Peter L Albertson, Mark D Harrison, William O S Doherty, Ian M O'Hara

Bioresour Technol. 2015 Sep;192:367-73.

PMID: 26056778

Abstract:

In this study, for the first time the effects of glycerol on enzymatic hydrolysis and ethanol fermentation were investigated. Enzymatic hydrolysis was inhibited slightly with 2.0 wt% glycerol, leading to reduction in glucan digestibility from 84.9% without glycerol to 82.9% (72 h). With 5.0 wt% and 10.0 wt% glycerol, glucan digestibility was reduced by 4.5% and 11.0%, respectively. However, glycerol did not irreversibly inhibit cellulase enzymes. Ethanol fermentation was not affected by glycerol up to 5.0 wt%, but was inhibited slightly at 10.0 wt% glycerol, resulting in reduction in ethanol yield from 86.0% in the absence of glycerol to 83.7% (20 h). Based on the results of laboratory and pilot-scale experiments, it was estimated that 0.142 kg ethanol can be produced from 1.0 kg dry bagasse (a glucan content of 38.0%) after pretreatment with acidified glycerol solution.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP56815-A	Glycerol solution		56-81-5	Price

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