Home
Resources
Publications
Effects of Histone Deacetylase Inhibitor Oxamflatin on in Vitro Porcine Somatic Cell Nuclear Transfer Embryos

Effects of Histone Deacetylase Inhibitor Oxamflatin on in Vitro Porcine Somatic Cell Nuclear Transfer Embryos

Liming Hou, Fanhua Ma, Jinzeng Yang, Hasan Riaz, Yongliang Wang, Wangjun Wu, Xiaoliang Xia, Zhiyuan Ma, Ying Zhou, Lin Zhang, Wenqin Ying, Dequan Xu, Bo Zuo, Zhuqing Ren, Yuanzhu Xiong

Cell Reprogram. 2014 Aug;16(4):253-65.

PMID: 24960409

Abstract:

Low cloning efficiency is considered to be caused by the incomplete or aberrant epigenetic reprogramming of differentiated donor cells in somatic cell nuclear transfer (SCNT) embryos. Oxamflatin, a novel class of histone deacetylase inhibitor (HDACi), has been found to improve the in vitro and full-term developmental potential of SCNT embryos. In the present study, we studied the effects of oxamflatin treatment on in vitro porcine SCNT embryos. Our results indicated that the rate of in vitro blastocyst formation of SCNT embryos treated with 1 μM oxamflatin for 15 h postactivation was significantly higher than all other treatments. Treatment of oxamflatin decreased the relative histone deacetylase (HDAC) activity in cloned embryos and resulted in hyperacetylation levels of histone H3 at lysine 9 (AcH3K9) and histone H4 at lysine 5 (AcH4K5) at pronuclear, two-cell, and four-cell stages partly through downregulating HDAC1. The suppression of HDAC6 through oxamflatin increased the nonhistone acetylation level of α-tubulin during the mitotic cell cycle of early SCNT embryos. In addition, we demonstrated that oxamflatin downregulated DNA methyltransferase 1 (DNMT1) expression and global DNA methylation level (5-methylcytosine) in two-cell-stage porcine SCNT embryos. The pluripotency-related gene POU5F1 was found to be upregulated in the oxamflatin-treated group with a decreased DNA methylation tendency in its promoter regions. Treatment of oxamflatin did not change the locus-specific DNA methylation levels of Sus scrofa heterochromatic satellite DNA sequences at the blastocyst stage. Meanwhile, our findings suggest that treatment with HDACi may contribute to maintaining the stable status of cytoskeleton-associated elements, such as acetylated α-tubulin, which may be the crucial determinants of donor nuclear reprogramming in early SCNT embryos. In summary, oxamflatin treatment improves the developmental potential of porcine SCNT embryos in vitro.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP151720433	Oxamflatin		151720-43-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: