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Effects of Inhibition of Phosphodiesterase 3B in Pancreatic Islets on Insulin Secretion: A Potential Link With Some Stimulatory Pathways

Effects of Inhibition of Phosphodiesterase 3B in Pancreatic Islets on Insulin Secretion: A Potential Link With Some Stimulatory Pathways

Agnieszka Kilanowska, Tomasz Szkudelski

Arch Physiol Biochem. 2019 Jun 26;1-8.

PMID: 31240952

Abstract:

Objective: Elevated intracellular cAMP concentrations potentiate insulin secretion from pancreatic β cells. Phosphodiesterase 3B (PDE3B) is highly expressed in these cells and plays a role in the regulation of insulin secretion. Materials and methods: In this study, effects of amrinone, an inhibitor of PDE3B on insulin release from isolated pancreatic islets, were determined. Results: Exposure of islets to amrinone for 15, 30 and 90 min markedly increased secretion induced by 6.7 mM glucose. Amrinone enhanced also secretion stimulated by 6.7 mM glucose and DB-cAMP, an activator of PKA. It was also demonstrated that amrinone potentiated insulin secretion induced by 6.7 mM glucose in the combination with PMA (activator of PKC) or acetylcholine. However, the insulin-secretory response to glucose and glibenclamide was unchanged by amrinone. Conclusions: These results indicate that amrinone is capable of increasing insulin secretion; however, its action is restricted.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP60719848	Amrinone		60719-84-8	Price

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