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Effects of Modification of Amino Groups With Poly(ethylene Glycol) on a Recombinant Uricase From Bacillus Fastidiosus

Chun Zhang, Xiaolan Yang, Juan Feng, Yonghua Yuan, Xiang Li, Youquan Bu, Yanling Xie, Huidong Yuan, Fei Liao

Biosci Biotechnol Biochem. 2010;74(6):1298-301.

PMID: 20530883

Abstract:

After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9002124 Uricase from Bacillus fastidiosus Uricase from Bacillus fastidiosus 9002-12-4 Price
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